The Antibody Service provides a centralized resource for the production, purification and labeling of monoclonal antibodies. It also maintains a collection of hybridomas, purified and coupled antibodies for IGC investigators.
The Antibody Service offers the following procedures:
Quality Control of hybridomas:
- Thawing, expanding, freezing
- Mycoplasma testing and cleaning
- Quantification of Ig production by ELISA
Small to Medium scale Ig production from QC hybridomas in vitro (10 to 100mg):
- Optimization of production by sub-cloning
- Adaptation to serum free or IgG depleted media
- Purification by Protein A/G chromatography and quantification
- QC by protein gel electrophoresis
Conjugation of monoclonal antibodies to small molecules for FACS, western or immunohistology
