The Leica EM AFS2 is a specially designed tool dedicated to low temperature sample fixation and resin embedding. By doing specimen preparation at low temperatures, artifacts such as sample shrinkage, membrane movement and sample extraction are greatly reduced which compared to specimens processed by standard electron microscopy protocols.
The Leica AFS2 is equipped with a stereomicroscope, fume exhaust system for added safety, fast LED UV polymerization for Lowicryl resin polymerization, and an intuitive programmable interface. For processing protocols that do not require the use of Osmium Tetroxide, an automated robotic system is available to do all solution changes.
Freeze substitution protocols for bacteria, tissue culture cells, cultured cells grown on sapphire dics and samples for correlative light and electron microscopy as well as immunogold labeling have been tested and implemented by the electron microscopy facility.
The Leica EM AFS2 is located in a dedicated floor mounted fumehood close to the High Pressure Freezer. Please contact one of the technicians in the IGC Electron Microscopy Facility for specialized training and experiment planning. The Leica AFS2 can be reserved here.