Carbon/Metal Coating: this feature provides the possibility of creating carbon films by carbon filaments or carbon rods. This is commonly used in Electron Microscopy to deposit carbon on formvar films which increases electrical conductivity and mechanical stability. Carbon coating is used frequently for sections that will have extensive exposure to the electron beam. The carbon layer helps reduce image drift in the short term and section thinning in the long term. Carbon coating is also used to prepare grids for negative staining and to prepare landmarks on surfaces used for correlative light and electron microscopy.
Carbon Coating with a Thin Film Monitor: this combination of tools allows you to deposite carbon at a known and pre-defined thickness.
Glow Discharge: using glow discharge hydrophobic surfaces (such as those created during carbon coating) can be turned into hydrophilic surfaces. This is used frequently in the preparation of samples for correlative light and electron microscopy (CLEM) as well as for grid preparation just prior to negative staining.
Sputter Coating: allows you to create a thin electrically conductive metal layer, on the surface of your sample. This is useful when you are preparing your specimens for Scanning Electron Microscopy (SEM).
The Q150T ES Carbon Coater needs specialized training that can be provided by one of the technical team in the IGC Electron Microscopy Facility. The training will predominantly introduce the user to the different standardized programs as well as explain to the user how to mount the various interchangeable inserts.
To book this equipment, you can do it here. If you have further questions please contact the IGC Electron Microscopy Facility technical team.