No single microscopy technique is capable of providing all of the desired information. Fluorescence light microscopy offers the rapid screening of a large area and it is advantageous to see the living, functional and dynamic cell, however, to study the details of the ultrastructure at a high-resolution, electron microscopy is needed.
Correlative Light Electron Microscopy (CLEM) is a powerful technique that combines the two different imaging methods, light microscopy and electron microscopy, and has been developed to take advantage of the functionality of both imaging techniques.
CLEM strategies, with the dual advantages of fluorescent imaging with the high-resolution of electron microscopy, are the most-informative approach allowing a direct correlation of the data sets obtained from one sample in two different microscopy techniques. CLEM is ideally suited for finding rare events that would be very hard to locate and identify using conventional electron microscopy screening.
This technique can be used in combination with immuno markers for studying cell structure and protein localization and can provide additional tools for three-dimensional analysis if combined with electron tomography.
If you have further questions please contact one of the technicians in the IGC Electron Microscopy Facility.