Tecnai G2 Spirit BioTWIN from FEI

  • The Tecnai G2 Spirit BioTWIN Transmission Electron Microscope (TEM) from FEI was installed at the Instituto Gulbenkian de Ciência in April 2016.

    This microscope has a high-contrast BioTWIN objective lens optimized for 2D and 3D data acquisition of biological samples. The automatic Compustage and advanced software enable overnight data acquisitions of electron tomography data to be done.

    The Tecnai Spirit Bio-Twin is equipped with the iCorr module, an integrated light microscope that can be used for correlative light and electron microscopy. The specifications for this feature are:

  • Objective lens 15x (0.5NA) (lateral resolution = 0.7µm)
  • Excitation 465-500nm, peak at 470
  • Emission 515-560 nm
  • The iCorr Camera is a 5 mega pixel, low-noise CCD camera. The physical pixel size of the camera chip is 3.45 µm.

The Tecnai Spirit Bio-Twin is equipped with two cameras. Camera 1 is the Olympus-SIS Veleta CCD Camera, a side mount 2k x 2k camera that is optimal for rapid screening of the sample. Camera 2 is an Eagle 4K HS camera, a bottom mount 4k x 4k camera for high resolution data acquisition of sensitive samples.

The Tecnai G2 needs special training that can be provided by one of the technicians in the IGC Electron Microscopy Facility or we can help you with your data acquisiton. Please contact us to learn about usage options.

To book this equipment, you can do it here. Booking slots are limited to 3 hrs for regular data acquisition during normal facility operation which is from 09:30 - 17:30. If you require longer periods of time on the microscope this can be arranged in advance with the facility staff.

If you have further questions please contact one of the technicians in the IGC Electron Microscopy Facility.