IGC

Immunolabeling

Immunolabeling is a technique that labels a protein of interest with an electron dense indicator that can be observed in the electron microscope. We typically use a gold bead ranging in size from 5-20nm as the electron dense indicator.

There are multiple ways of preparing the sample for immunolabeling such as the Tokuyasu Technique, High Pressure Freezing followed by Automatic Freeze Substitution or even EPON section embedding. All of these techniques can be done in the Instituto Gulbenkian de Ciência Electron Microscopy Facility. That being said, it is a challenging technique and the feasibility of each project needs to be carefully assessed.

Please contact one of the technicians in the IGC Electron Microscopy Facility if you are interested in Immunolabeling protocols.