The Leica UC7 / FC7 Ultramicrotome was installed in the EM Facility in February 2015. The instrument is in an isolated room, free from air currents and constant interruptions. This instrument is used for sample trimming, and cutting of ultra-thin sections (50-90 nm), and semi-thin sections (300 – 700 nm) at both room and cryo temperatures. A section thickness of 60 – 120 nm is optimal for TEM imaging on the Hitachi H-7650 located in the electron microscopy facility.
The ultramicrotome room is equipped with an oxygen sensor as a safety measure for handling liquid nitrogen during cryo-sectioning. The instrument is equipped with a cryo-sphere and a micromanipulator system to improve the quality and easy of cryo-sectioning.
Cryo-sectioning has priority on this instrument, however it is also available for use on room temperature samples.
In order to book this equipment, you can do it here. Booking slots are limited to 3-hrs for room temperature sectioning (9:00 am -12:00 pm, 12:00 pm – 3:00 pm, and 3:00 pm – 6:00 pm) or all day for cryo-sectioning.
If you have further questions please contact one of the technicians in the IGC Electron Microscopy Facility.