Negative staining is an electron microscopy technique used to study shape, size and organization of small structures, such as proteins, viruses, bacteria or parasites.
Negative staining is done by contrasting the thin specimen with electron dense salts (like uranyl acetate, or phosphotungstic acid) and it is the simplest of all electron microscopy techniques to learn. The electron dense stain will surround the structure, but it will not penetrate the specimen. The result reveals the limit of the specimen and surface details. In some cases it can be used in conjunction with Immunolabeling.
Please contact one of the technicians in the IGC Electron Microscopy Facility if you are interested in Negative Staining.