The Olympus IX81 inverted fluorescence microscope is equipped with a Cascade CCD digital camera. The microscope has long working 20x and 40x air objectives and 40x, 60x and 100x oil immersion objectives. The microscope is equipped with polarizing and Wollaston optics enabling DIC microscopy and it has four fluorescence filter sets for epi-fluorescence microscopy: F36-513 (DAPI), F36-525 (GFP), F36-504 (TxRed) and F46-009 (Cy5). See here for more details.
The fluorescence microscope is located adjacent to the high pressure freezer so that samples can be imaged just prior to high-pressure freezing for Correlative Light and Electron Microscopy (CLEM) techniques.
To book this equipment, you can do it here. Please contact one of the technicians in the IGC Electron Microscopy Facility for in-depth experiment planning and training on the Olympus IX81 inverted fluorescence microscope.
If you are interested in learning more about the CLEM technique please contact Dra Erin Tranfield.
This microscope was kindly donated to the Electron Microscopy Facility by the European Molecular Biology Laboratory in Heidelberg Germany.