IGC

Electron Microscopy Facility Staff

If you are interested in using the IGC Electron Microscopy Facility, your first step is to contact Dra. Erin Tranfield for an EM Consultation.

  • Erin Tranfield

    Erin Tranfield, PhD

    Electron Microscopy Facility Head

    Email: etranfield@igc.gulbenkian.pt

    Phone: +351 214 464 691

    Internal Ext: 4691

  • Erin Tranfield obtained her PhD at the University of British Columbia in Vancouver, Canada. Her thesis focused on the ultrastructural changes of atherosclerotic plaques following exposure to fine particulate air pollution. Her first post-doctoral fellowship was at NASA Ames Research Center in Moffett Field, USA where she studied the toxicity of lunar dust. Her second post-doctoral fellowship was at The European Molecular Biology Laboratory (EMBL) in Heidelberg Germany where she developed protocols to study the ultrastructure of the meiotic spindle of Xenopus laevis. Erin joined the Instituto Gulbenkian de Ciência in 2013 to first build and then run the Electron Microscopy Facility.

    Erin has be doing electron microscopy since 1999, and her expertise lies in the areas of high pressure freezing and automatic freeze substitution, electron tomography and three-dimensional data acquisition as well as Correlative Light and Electron Microscopy (CLEM). Within the Electron Microscopy Facility, Erin works with the technical staff to train users, maintain equipment and to determine the protocols needed to best address the scientific questions of a given project.

    If you have any questions about the equipment available in the EM Facility, about the opportunity to use the facility or potential collaborations please contract Dra. Erin Tranfield. If you are interested in tours of the facility for schools or interested groups, please refer to Science at Schools.


  • Ana Laura Sousa

    Ana Laura Sousa, M.Sc.

    Electron Microscopy Technician

    Email: alsousa@igc.gulbenkian.pt

    Phone: + 351 214 464 676

    Internal Ext: 4676

  • Ana Laura graduated in Biomedical Sciences - Histocellular Pathology at Escola Superior de Tecnologia da Saúde de Lisboa. During the degree she developed research projects and participated in several practical internships, including Histopathology and Electron Microscopy applied to Clinical Diagnosis. Ana finished her Masters Degree in November 2017.

    Ana has worked as an Electron Microscopy Technician at the Instituto Gulbenkian de Ciencia since 2013, and she has mastered the art of biological sample processing, sample analysis and operating both the Tecnai G2 Spirit BioTWIN TEM and the Hitachi TEM. She has developed multiple Correlative Light and Electron Microscopy Workflows and she is well respected within the European Community for her skill and technical expertise in these areas.

    Ana's Facility Responsibilities Include:

    • Technical assistance with experimental planning and sample preparation;
    • Protocol Optimization;
    • Chemical Fixation;
    • Resin Embedding;
    • Sectioning and Cryosectioning - Glass and Diamond;
    • Immunolabeling;
    • Negative Staining;
    • Two and Three Dimensional Imaging;
    • Correlative Light and Electron Microscopy (CLEM) Workflows;
    • Training and Technical assistance for users of the Facility.

    Key Activities: CLEM and Tokuyasu Techniques.


  • Maria João Almeida

    Maria João Almeida, M.Sc.

    Electron Microscopy Technician

    Email: mjalmeida@igc.gulbenkian.pt

    Phone: + 351 214 464 676

    Internal Ext: 4676

  • Maria João has a degree in Environmental Biology from the Faculdade de Ciências da Universidade de Lisboa and finished her Masters degree in 2009 at the same institution. Since then, she has mostly worked in scientific projects about environmental quality evaluation, and has taught high school and university students through different mentoring programs.

    In January 2020, Maria João joined the EM Facility team and works as an Electron Microscopy technician at the Instituto Gulbenkian de Ciência, processing and analyzing biological samples and operating the Hitachi TEM and the Tecnai G2 Spirit BioTWIN TEM.

    Maria's Facility Responsibilities Include:

    • Experimental planning and sample preparation;
    • Protocol Optimization;
    • Chemical Fixation and Resin Embedding;
    • Sample sectioning - Glass and Diamond;
    • Negative Staining;
    • Two Dimensional Imaging;
    • Training and Technical assistance for users of the Facility.

    Key Activities: Negative Staining, Sample Sectioning, and Conventional sample preparational.


  • Ana Sofia Pacheco

    Ana Sofia Pacheco, B.Sc.

    Electron Microscopy Technician

    Email: spacheco@igc.gulbenkian.pt

    Phone: + 351 214 464 676

    Internal Ext: 4676

  • Sofia has a degree in laboratory biomedical sciences from the Escola Superior de Saúde de Bragança. During the degree, she had internships in different areas like Histology, Microbiology, Biochemistry, Molecular Biology. After the course, she worked for 1 year as a technician in electron microscopy applied to material sciences.

    In January 2020, Sofia joined the Instituto Gulbenkian Ciência as an electron microscopy technician preparing the samples and collecting data on the microscopes available in the facility.

    Sofia's Facility Responsibilities Include:

    • Experimental planning and sample preparation;
    • Protocol Optimization;
    • Chemical Fixation and Resin Embedding;
    • Sample sectioning - Glass and Diamond;
    • Negative Staining of biological and material science samples;
    • Two Dimensional Imaging;
    • Training and Technical assistance for users of the Facility.

    Key Activities: Negative Staining, Sample sectioning, and Conventional sample preparational.