IGC

Electron Microscopy Facility Staff

If you are interested in using the IGC Electron Microscopy Facility, your first step is to contact Dra. Erin Tranfield for an EM Consultation.

  • Erin Tranfield

    Erin Tranfield, PhD

    Head of the Electron Microscopy Facility

    Email: etranfield@igc.gulbenkian.pt

    Phone: +351 214 464 691

    Ext: 4691

  • Erin Tranfield obtained her PhD at the University of British Columbia in Vancouver, Canada. Her thesis focused on the ultrastructural changes of atherosclerotic plaques following exposure to fine particulate air pollution. Her first post-doctoral fellowship was at NASA Ames Research Center in Moffett Field, USA where she studied the toxicity of lunar dust. Her second post-doctoral fellowship was at The European Molecular Biology Laboratory (EMBL) in Heidelberg Germany where she developed protocols to study the ultrastructure of the meiotic spindle of Xenopus laevis. Erin joined the Instituto Gulbenkian de Ciência in 2013 to first build and then run the Electron Microscopy Facility.

    Erin has more than 15 years experience with electron microscopy, particularly high pressure freezing and automatic freeze substitution, electron tomography and three-dimensional data acquisition. Within the Electron Microscopy Facility, Erin works with the technical staff to train users, maintain equipment and to determine the protocols needed to best address the scientific questions of a given project.

    If you have any questions about the equipment available in the EM Facility, about the opportunity to use the facility or potential collaborations please contract Dra. Erin Tranfield. If you are interested in tours of the facility for schools or interested groups, please refer to Science at Schools.

Facility Technicians

  • Ana Laura Sousa

    Ana Laura Sousa, B.Sc.

    Electron Microscopy Technician

    Email: alsousa@igc.gulbenkian.pt

    Phone: + 351 214 464 676

    Ext: 4676

  • Ana Laura graduated in Biomedical Sciences – Histocellular Pathology at Escola Superior de Tecnologia da Saúde de Lisboa. During the degree she developed research projects and participated in several practical internships, including Histopathology and Electron Microscopy applied to Clinical Diagnosis.

    Nowadays, Ana works as an Electron Microscopy Technician at the Instituto Gulbenkian de Ciencia, processing and analyzing biological samples and operating the Hitachi TEM. Ana Laura is also currently working on her Masters Degree.

    Her responsibilities cover:

    • Technical assistance with experimental planning and sample preparation;
    • Protocol Optimization;
    • Chemical Fixation;
    • Resin Embedding;
    • Sectioning and Cryosectioning – Glass and Diamond;
    • Immunolabeling;
    • Negative Staining;
    • Two and Three Dimensional Imaging;
    • Training and Technical assistance for users of the Facility.

    Key Activities: CLEM (Correlative Light Electron Microscopy) and Tokuyasu Techniques.


  • Sara Bonucci Costa

    Sara Bonucci Costa, M.Sc.

    Electron Microscopy Technician

    Email: sbcosta@igc.gulbenkian.pt

    Phone: + 351 214 464 676

    Ext: 4676

  • Sara Bonucci graduated in Biochemistry from the Faculty of Sciences, at the University of Lisbon. During the degree she had extensive practical training, allowing her to gain detailed knowledge for many laboratories techniques. Sara completed a final paper focusing on Biochemistry Regulation

    For the Masters degree in Human Biology and Environment, also at the Faculty of Sciences, she did her thesis in the Neurotoxicology research of a chemical compound (n-hexane).

    Sara is an Electron Microscopy Technician at the Instituto Gulbenkian da Ciência where she is applying her biochemistry background to protocol optimization.

    Her responsibilities cover:

    • Technical assistance with experimental planning and sample preparation;;
    • Chemical Fixation;
    • Plastic Embedding;
    • Sample Sectioning – Glass and Diamond;
    • Training and Technical assistance for users of the Facility;
    • Negative staining;
    • Cryo Techniques (High Pressure Freezer; Freeze substitution).

    Key Activities: Protocol Optimization; High Pressure Freezer; Freeze Substitution.