We have currently 2 cell sorters available, each with different assets. The cell sorters are operated by the FCF staff, and as such it is dependent not only of the machine availability, but also the staff availability. To schedule an experiment talk to the FCF staff.
BD FACSAria IIu is equipped with 3 different lasers that can be used simultaneously. It can sort up to 20 000 events per second with high precision.
It can sort 4 populations at the same time to eppendorfs, 5mL tubes or PCR tubes, and 2 populations at the same time for 15mL falcons. It can also sort to PCR strips, 6/96/384-wells plates, to terazaki plates or to slides (one population at a time). It is installed in a security chamber which allows us to sort human samples with biological risk (BSL2). It comes with 3 nozzles with different sizes (70, 85 and 100 µm) that we can change depending on the samples.
NOTE: Besides the fluorochromes mentioned above, we have filters that enable us to read BV605, BV711 ou BV785. Be aware that Aria can only read 2 colors in Violet simultaneously. If you need to use these colors, contact the FCF staff.
Moflo is equipped with 4 different lasers, although the Multiline (514 nm) and the Yellow Green (561 nm) lasers can't be used simultaneously.
It can sort 3 populations at the same time to epperdorfs and 5mL tubes. It can also sort to 6/96-wells plates or to slides (one population at a time). It comes with 2 nozzles with different sizes (70 and 100 µm) that we can change depending on the samples.
The use of the sorters is paid per hour of reservation.