FACSCalibur is a robust 2-laser 4-Color flow cytometry Analyzer produced by Becton Dickinson. Manufactured in 1997, it has been continuously serviced by BD (represented by Enzifarma), with a couple of regular checkups each year. In addition, optical quality control and calibration are performed weekly, and due to heavy usage of the machine, daily cleanings and weekly long cleans are performed. Typical fluorescent labels detected are FITC, Alexa488, GFP, CFSE, YFP, PE, PerCP, PE-Cy5, PI, 7-AAD, APC, Cy5, etc). For more information, check the following topics:
CyAn ADP is equipped with a 25mW solid state blue (488nm) laser, a 50mW solid state violet (405nm) laser, and a 60mW red (643nm) diode. Note that a band-pass filter designated as 530/40 indicates that it will detect light in the a 30nm range centered at 530nm, i.e., 515-545nm. LP stands for Long Pass filter, and indicates that it will detect light above the mentioned wavelength. A list of typical fluorescent labels detected in each channel is also displayed on the right.
CyAn ADP is connected to a PC loaded with Summit 4.3 for machine interface and data acquisition. Acquired data is saved direclty on the hard drive. Use the D:\facsdata\ partition for this purpose. Due to space constraints, all data is routinely cleaned. To avoid data loss, the users MUST transfer their data to another location. The best way to store your own data is to transfer it to a pen-drive, USB external hard disk, or to your directory in xserve02. To transfer data to xserve02 or to another network location, follow these steps:
Our CyAn ADP has been custom changed to include a direct waste system eliminating the need for a waste tank. Therefore, users do not have to keep changing the waste tank when it's full. The sheath tank, however, must be ALWAYS refilled when the sheath indicator is orange, meaning the system is running low on sheath solution.