FACSCalibur is a robust 2-laser 4-Color flow cytometry Analyzer produced by Becton Dickinson. Manufactured in 1997, it has been continuously serviced by BD (represented by Enzifarma), with a couple of regular checkups each year. In addition, optical quality control and calibration are performed weekly, and due to heavy usage of the machine, daily cleanings and weekly long cleans are performed. Typical fluorescent labels detected are FITC, Alexa488, GFP, CFSE, YFP, PE, PerCP, PE-Cy5, PI, 7-AAD, APC, Cy5, etc). For more information, check the following topics:
FACSCalibur is equipped with a typical low-power aircooled 15mW blue (488nm) argon laser, from which 3 colors can be detected (FL1, FL2, and FL3), and a red (635nm) diode laser, which can detect APC or Cy5 (FL4). Therefore, 4 different colors can be detected simultaneously, based on the 2 excitation wavelengths and the wavelength range of each detector, shown in the figure below. Note that a band-pass filter designated as 530/30 indicates that it will detect light in the a 30nm range centered at 530nm, i.e., 515-545nm. LP stands for Long Pass filter, and indicates that it will detect light above the mentioned wavelength. A list of typical fluorescent labels detected in each channel is also displayed on the right.