LSR Fortessa X-20 SORP is a powerful bench cell analyzer from Becton-Dickinson, equipped with 4 lasers (blue, red, violet, and yellow flowcytometry), detecting up to 16 colors simultaneously. Flow cytometry data is analyzed using BD FACSDiva™ 8 software for an efficient setup, acquisition and analysis. For more information, check the following topics:
The X-20 is equipped with 4 different low-power lasers that can be used simultaneously. A 50mW solid-state blue (488nm) laser, from which SSC and 2 colors can be detected; a solid-state 40mW violet (446nm) laser with 6 detectors, a solid-state 40mW red (446nm) laser, from which 3 colors can be detected, and a 40mW yellow-flowcytometry (561nm) laser where 5 colors can be detected. Instead of calling the detection channels generically FL1-to-FL16, each channel is named after it's respective PMT detector, to easily setup an experiment users should label which fluorochromes are being detected. Note that a band-pass filter designated as 530/30 indicates that it will detect light in the a 30nm range centered at 530nm, i.e., 515-545nm.
Acquired data is saved directly on the hard drive. Use the D:\EXPORT - Folder routinely cleared\ partition for this purpose.
The way data is exported, depends on where the data is to be analyzed:
• ![endif]>export as Experiment if you are going to analyze on an offline FACSDiva software & wish to keep plots & gates
•export as FCS files to analyze data in any flow cytometry software
To avoid reaching the limit size of FACSDiva Database file, all Experiments must be exported after acquisition. Please confirm if files were successfully exported to your folder and DELETE them, leaving only an empty Experiment template within the software.
Due to space constraints, all data is routinely cleaned. To avoid data loss, the users MUST transfer their data to another location. The best way to store your own data is to transfer it to a pen-drive, USB external hard disk, or to your directory in remote server. To transfer data to filespc or to another network location, follow these steps: