LSR Fortessa SORP is a powerful bench cell analyzer from Becton-Dickinson, equipped with 3 lasers (violet, blue and yellow flowcytometry), detecting up to 13 colors simultaneously. Flow cytometry data is analyzed using BD FACSDiva™ software for an efficient setup, acquisition and analysis. Analysis can be made in tubes or in 96 well plates, using BD™ High Throughput Sampler (HTS). For more information, check the following topics:
The LSR Fortessa is equipped with 3 different low-power lasers that can be used simultaneously. A 50mW solid-state blue (488nm) laser, from which SSC and another 5 colors can be detected (upgradable to another 2); a 40mW yellow-flowcytometry (561nm) laser, from which 5 colors can be detected; and a solid-state 40mW violet (446nm) laser, from which 2 colors can be detected (also upgradable with an extra color). Instead of calling the detection channels generically FL1-FL9, BD named each channel after the most typical fluorochrome it detects. Note that a band-pass filter designated as 530/30 indicates that it will detect light in the a 30nm range centered at 530nm, i.e., 515-545nm. LP stands for Long Pass filter, and indicates that it will detect light above the mentioned wavelength.
Acquired data is saved directly on the hard drive. Use the D:\facsdata\ partition for this purpose.
The way data is exported, depends on where the data is to be analyzed:
• export as Experiment if you are going to analyze on an offline Diva software & wish to keep plots & gates
• export as FCS files to analyze data in any flow cytometry software
To avoid reaching the limit size of Diva Database file, all Experiments must be exported after acquisition. Please confirm if files were successfully exported to your folder and DELETE them, leaving only an empty Experiment template within the software.
Due to space constraints, all data is routinely cleaned. To avoid data loss, the users MUST transfer their data to another location. The best way to store your own data is to transfer it to a pen-drive, USB external hard disk, or to your directory in remote server. To transfer data to filespc or to another network location, follow these steps:
Our LSR Fortessa has been custom changed to allow the inspection of the instrument’s flow cell, easily allowing the examination for the existence of bubbles in the flow cell and also laser alignment.
The machine also includes a direct waste system eliminating the need for a waste tank. Therefore, users do not have to keep changing the waste tank when it's full. The sheath tank, however, must be ALWAYS refilled after usage, or if running low on PBS.