IGC

MAGPIX (Luminex / Merck) | Reservations

  • MAGPIX is an instrument that allows simultaneously, detecting and quantifying multiple proteins in a single biological sample. With this magnetic bead-based system is possible to measure up to 50 analytes in 25 µL of sample. The underlying technology remains one of the most applied in life sciences, including clinical diagnostics.

    Unlike flow cytometry which leverages a fluid flow to align beads single file through a cuvette, where they can be illuminated with lasers, the MAGPIX instrument flows beads with significantly less fluid volume into a chamber. There they are held in a monolayer with a magnet. LEDs are focused on the chamber to illuminate the beads, which are imaged using a CCD camera. Image processing algorithms are then run to deliver the assay results.

    For more information, check the following topics:

  • MAGPIX

Optical configuration

Operating Procedures

  • Before Acquisition

    1. If machine is turned OFF, please follow the next procedure:
      • Turn ON the PC
      • Turn ON the front power button of MAGPix
    2. Start xPONENT software and log in with your credentials (provided previously)
    3. Go to Maintenance page > Auto Maint tab and select the System Initialization procedure.
    4. Click Eject on the status bar, and fill the reservoirs with the correct solutions as exemplified in the screen.
    5. Click Retract, and then click Run.
    6. Open the Protocol page, then open the Protocols tab. Select your acquistion protocol or click Create New Protocol to assign Protocol name, Acquisition Settings, Analysis Settings (if quantitative analysis) and Plate Layout; you can then save the aquisition Batch. For more specific information see the provided User Quick Guide.
    7. Click Run Batch to begin acquisition.

    If you have any doubts or need assistance call the facility's staff (ext. 251)


  • After Acquisition

    1. Run Shutdown procedure. Go to Maintenance page > Auto Maint tab > System Shutdown.
    2. Click Eject on the status bar, and fill the reservoirs with the correct solutions as exemplified in the screen.
    3. Click Retract, and then click Run
    4. Open the Results page, then the Reports tab. Select the category of report: batch, protocol, or advance, then select the specific report from the Type list.
    5. Click Generate.
    6. Export your generated reports and REMOVE all your acquisition data from the hard drive The computer is NOT for data storage. The hard-disk is subject to routine cleaning, and files may be deleted without warning.

  • Trasfering Data

    MAGPix is connected to a PC loaded with xPONENT for machine interface and data acquisition. Acquired data is saved directly on the hard drive, see the C:\\Program Files\xPONENT\Output folder for this purpose. Due to space constraints, all data is routinely cleaned. To avoid data loss, the users MUST transfer their data to another location. The best way to store your own data is to transfer it to a pen-drive, USB external hard disk, or to your directory in filespc server. To transfer data to filespc or to another network location, follow these steps:

    1. Open any folder window.
    2. Write "\\filespc" on the location bar and hit Enter.
    3. You'll be prompted for your username and password.
    4. Select the folder you want to access.
    5. Locate and drag your local files into the remote folder.
    6. To disconnect from the remote drive you have to run one of the 'windows-logout' executable files located in the remote server.