In this section we provide several success ful protocols for sample preparation and cell sorting experiments.
We detail the conditions in which samples are prepared for sorting and how the machines are setup and run for cell separation.
Preparation for Cell Sorting
Antibody staining of surface antigens
Intracellular staining - PFA & saponin
Intracellular staining for Transcription Factors
Intracellular staining for cytokines
Cell Cycle protocol for FC
Yet to be finnished. If you have any questions, contact the FCF staff.