IGC

Protocols


In this section we provide several success ful protocols for sample preparation and cell sorting experiments. We detail the conditions in which samples are prepared for sorting and how the machines are setup and run for cell separation.



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Preparation for Cell Sorting


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Antibody staining of surface antigens

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Intracellular staining - PFA & saponin

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Intracellular staining for Transcription Factors

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Intracellular staining for cytokines


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Cell Cycle protocol for FC


Yet to be finnished. If you have any questions, contact the FCF staff.


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CFSE staining


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Ficoll Gradient
to isolate PBMCs

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Thermofisher's Cell Preparation Protocol

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Thermofisher's Viability Staining Protocol

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Thermofisher's Surface Staining Protocol


Other tools


Under construction...