IGC

Services : Long-Read Sequencing

We have been testing and developing long read DNA sequencing technology using the Oxford Nanopore MinION system since 2016.

Key features:

  • Long-read analysis.
  • Up to 512 nanopore channels.
  • Simple 10-min sample prep available.
  • Real-time analysis for rapid, efficient workflows.
  • Adaptable to direct DNA or RNA sequencing.

Requirements:

  • high quality, pure genomic DNA (gDNA).
  • urity as measured using Nanodrop – OD 260/280 of 1.8 and OD 260/230 of 2.0–2.2.
  • Average fragment size, as measured by pulsed-field gel analysis ( >30 kb)
  • Input mass, as measured by Qubit – 1 µg.