IGC

Services : Whole Genome Sequencing

We have implemented a low-volume Nextera protocol that uses the Tn5 transposase for tagmentation-based library construction.

Key features:

  • Low input, low cost.
  • Can also be used to sequence plasmids or amplicons (e.g. mix of PCR amplified genomic regions of interest).
  • Sequencing on NextSeq500 (e.g. 2x150bp PE) or MiSeq (e.g. 2x250bp PE).

Requirements:

  • 1 ng of high quality genomic DNA or amplicon(s) larger than 400bp.