The HU, benefiting from a fully equipped laboratory, provides the following services:
(Performed by our own technicians or on a self-service basis, except processing, paraffin em-bedding and microtomy - not available for self-service).
- Processing and Paraffin Embedding
- Microtome Sectioning
- Cryostat sectioning
- Vibratome sectioning
- H&E staining and special staining (such as Gram, Giemsa, Ziehl-Neelsen, PAS, Luxol Fast Blue with Nissl or PAS contrast, Masson's Trichrome, Nissl, Perls, Carstair's Method, Luna stain and others)
- Training for new users in sample preparation
- Training for new users in cryostat sectioning
- Training for new users in vibratome sectioning
- Planning and implementation of different and new histological techniques including Immunohistochemistry
- Project discussion with the Pathologist
- Results analysis
How to use the service
- Technical Service Requests: should be made through the HelpDesk by opening a task and filling the "Service Request Form" (SRF) when delivering samples or when request-ing counseling. (https://webint.igc.gulbenkian.pt/helpdesk/). Help in completing the SRF can be provided for first time users;
- Cryostat and vibratome: usage requires booking online. Induction/training is mandato-ry for first time users before obtaining booking permission (https://agendo.cirklo.org/igc/);
- Pathologist consultation: People are strongly encouraged to book a consultation with our Pathologist (Alistair Robson) prior to the planning of a project that involves histo-logical analysis. The project's aims and methods can be discussed and recommenda-tions made in order to successfully address the research question of interest. Online requisition should be properly filled and submitted prior to sample delivery (https://webint.igc.gulbenkian.pt/helpdesk/);
Similarly, researchers can schedule appointments for guidance in the histological in-terpretation of their samples (https://webint.igc.gulbenkian.pt/helpdesk/). Finally, fol-lowing completion of the project, the results and/or any issues that arose in the final histological analysis can be reviewed.
- Users should provide boxes for storage of unstained slides when delivering samples.
- Researchers will be notified by e-mail, through the HelpDesk, as soon as the work is completed, and the slides can be collected from the technicians in the lab.
- Material should not be stored at the HU for more than 30 days.
- When the work is for pathology assessment the slides will be immediately re-directed to the pathologist (be sure to include all details regarding experimental setting and ex-pected outcome of the pathology assessment in the request form or contact the pathologist).