IGC

Nikon E400 | Reservations

  • Nikon E400 Phase Contrast Microscope
  • The Nikon E400 is an upright microscope for experiments with Brightfield and Phase Contrast.

    Phase contrast is a technique to improve contrast in thin samples. You will have seen it in the inverted microscopes used in the cell culture rooms. It is very useful to identify small, flat features such as adherent cells. It was one of the first contrast techniques developed and still is very useful. The Nikon E400 has been coupled with a color camera to be able to record images of histology preparations, cells and small organisms.

    Location Room : Gil Eanes

    Manufacturer : Nikon

    Model : E400

Suggestion for description in "Materials and Methods":

Images were acquired on a Leica DM LB2 upright microscope, equiped with a IDS color CCD camera, using the a 40x 0.75NA objetive and DIC optics, using the IrfanView software.


Available objectives

Magnification
Objective type
NA
Immersion
Working distance (mm)
Pixel size (µm)
Phase ann.
10x
PLAN
0.25
AIR
10.5
0,436 um
Ph1
20x
PLAN
0.40
AIR
1.3
0,218 um
Ph1
40x
PLAN
0.65
AIR
0.57
0,11 um
Ph2
100x
PLAN
1.25
OIL
0.17
0,044 um
Ph3

Setup

Turn on procedures

  1. Confirm your entry on the reservation system.
  2. Turn on the microscope
  3. Log on into the computer if it isn’t already logged on, the generic username is igcuser and password igc!usr.
  4. Start the Camera software available both as a Desktop shortcut and on the Windows start menu.
  5. On the camera software, find the "Snapshopt" button. You can then save images as a BMP or JPEG.

Turn off procedures

If you used any immersion lenses be sure to clean them.

TAKE OUT EVERYTHING YOU BROUGHT!, eg, pipettes, slides, etc...


Phase contrast

In order to perform phase contrast experiments you should first align the condenser for Kolher illumination.

Video explaining the procedure

For each objective you should match the Phase annulus on the condenser (see the image below) with the setting written on the objective or the one on the table above.

brightness


Extra Info

Transmitted light - Brigthfield

Phase Contrast