Error Status code: 401
The Zeiss' Stereo Lumar is a flurescence capable and fully automated stereoscope with an increased focusing range, which allows imaging of whole small animals (eg Zebrafish e c elegans), as well as cell cultures in petri dishes. Magnifications range is equivalent to ~1-12x, with variable zoom.
Location: Bartolomeu Dias Wing
Microscope: Zeiss SteREO Lumar.V12
Year Installed: 2011
Optics: Fluorescence and off-axis illumination
Cameras: Hamamatsu Orca-Flash (2.8MPx) and IDS uEye UI-3370CP (4.19MPx)
Operating conditions: Room Temperature
Options: None
Keywords:
Images were acquired on Zeiss STereo LUMAR stereoscope, equiped with a Hamamatsu Orca-Flash 2.8 camera and GFP fluorescence filterset, controlled with the MicroManager v1.14 software.
We would like to thank the technical support of IGC's Advanced Imaging Facility (AIF-UIC), which is supported by the national Portuguese funding ref# PPBI-POCI-01-0145-FEDER-022122, co-financed by Lisboa Regional Operational Programme (Lisboa 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) and Fundação para a Ciência e a Tecnologia (FCT; Portugal).
Name |
Excitation |
Emission |
CFP |
436/20 |
480/40 |
YFP |
500/20 |
535/30 |
GFP |
470/40 |
525/50 |
TxRed |
560/40 |
630/75 |
Because this stereoscope has a continuous zoom it is important that you write down the magnification at which the images were acquired (as displayed on the microscope controler). TO calibrate the images in ImageJ or any other software you need to insert the correct pixel size by using the calculator below.
Close all programs and Log-off from Windows.
On Micro-Manager press "Live" to get a live feed of your sample.