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IGC

AdvancedImagingUIC

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Hamamatsu Aequoria | Reservations

  • Aequoria

  • The Aequoria MDS system is a macroscopic darkbox system equiped with an EMCCD camera capable of acquiring faint luminescence signals in large samples such as whole living organisms, bacterial plates, plants or micro-well plates.

    Location:

    Manufacturer : Hamamatsu

    Model : Aequoria

    Year Installed: 2009

    Keywords:

Suggestion for description in "Materials and Methods":

Still yet to be updated. Soon.

Suggestion for "Acknowledgments":

We would like to thank the technical support of IGC's Advanced Imaging Facility (AIF-UIC), which is supported by the national Portuguese funding ref# PPBI-POCI-01-0145-FEDER-022122, co-financed by Lisboa Regional Operational Programme (Lisboa 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) and Fundação para a Ciência e a Tecnologia (FCT; Portugal).

Practical examples:

  • Quantification of Luciferase, Susana Ramos (Inflamation lab) | PDF | Direct Link

Setup

Turning on

  1. Turn on the computer.
  2. Log on into the computer if it isn’t already logged on, using the generic igcuser credentials.

  3. Turn on the camera controller.

    camera on/off switch

  4. Start MicroManager or HCImage software,both available as a Desktop shortcut or at the Windows start menu.

  5. Set up your experiments in the chamber.

    dark chamber

  6. Set exposure, Gain, Sensitivity and/or binning adequate to your sample.

Turning OFF

  • Save all your acquired pictures to the server/hard drive.
  • Close the acquisition software.
  • Turn off the camera controller box. ONLY IF THERE IS NO ONE COMING IN THE NEXT 2 HOURS.
  • TAKE OUT EVERYTHING YOU BROUGHT!, eg, pipettes, slides, etc...

If you would like to use it,just ask the UIC staff.

Extra Info