The Advanced Imaging Unit currently has a few microscopes divided in four categories.
Zeiss' Stereo Lumar is a fluorescence capable and fully automated stereoscope with an increased focusing range, letting you take pictures of tissues of whole adult fish, mice and even larger animals. With it's high resolution, high quality ApoLumar 1.2 lens, variable zoom and large working distance, users have flexibility to choose between their desired zoom without sacrificing quality.
The Aequoria MDS system is a macroscopic system to acquire luminescence in large samples like living large organisms, bacterial plates, plants or well plates, making it suitable for high-throughput screens. With an EMCCD chip it can detect the smallest signals on your samples with ease.
The Nikon HCS is an inverted widefield microscope with complete automation possibility. This microscope allows users to acquire large amounts of data with ease by giving instructions to the software at the beginning and it will do the rest. It is equipped with a sCMOS Andor Zyla camera with a large field of view and a chip size of 2048x2048, with both high and low magnification objectives for a quick screen with low magnification and then a higher resolution image after object identification.
The Leica HCScreening is an inverted widefield microscope with an automated stage and fast sets of filter wheels that allow automatic image acquisition (screening) and large field tile/grid stitching through a fast acquisition protocol. This microscope is prepared for automatically acquiring images at a lower magnification, where it will identify an object or structure of interest, and then acquire them at a higher and better resolution magnification.
The Zeiss Imager Z2 + Apotome is a fully automated upright microscope equipped with two cameras: A color camera (used for brighfield) and a monochromatic camera (for fluorescence). The microscope is equipped for fluorescence, differential interference contrast (DIC) and darkfield. With a fully motorized stage (XYZ), the system is capable of z-stacks, multiple positions and tile & stitching acquisition. The imager is also equipped with the ApoTome.2, a module that allows optical sectioning of fluorescent samples using structured illumination. Despite the need to process apotome images, it is still faster than a laser scanning confocal, so it should be tested as an alternative for thin samples that require a higher throughput than that possible with the SP5 and LSM980.
The 3i Marianas system is a spinning disk laser confocal microscope with a back-illuminated sCMOS camera suitable for fast in vivo imaging. It's the fastest and most sensitive system at the institute. The system can also scan multiple positions in the sample, with multiple channels in timelapse mode. The spinning disk unit comes coupled with a uniformizer (which uniformizes the illumination for the whole field of view), a laser ablation and FRAP system.
This confocal microscope allows acquisition of images at very high frame rates with minimum illumination of samples in a broader field of view, when compared with other spinning disks.
The Leica Spinning Disk system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in vivo imaging. It's the fastest and most sensitive system at the institute. The system can also scan multiple positions in the sample, with multiple channels in timelapse mode. Also has FRAP and Ablation systems.
The Leica Stellaris 5 system is mounted on a Leica DM6 upright microscope, equipped with 405 nm, 448 nm, 488 nm, 561 nm and 638 nm laser lines, and with two of Leica’s new Power HyD S spectral detectors. The Stellaris5 is equipped also to perform FRET (CFP-YFP AB or SE), FRAP, and Lightning (Super-resolution).
A multi-photon, or two-photon, microscope uses instrumentation similar to that of a laser scanning confocal microscope: A laser source for sample excitation, a scanhead with galvanometer controlled mirrors (or acousto-optic deflectors) to scan the excitation beam, and photomultiplier tubes to detect fluorescent signals. However, the confocal image differs in that optical sectioning is obtained using excitation in multi-photon microscopy whereas in confocal microscopy it is achieved using the emission pinhole.
The Zeiss LSM 980 is an inverted microscope equipped with a laser scanning head with a total of 5 detectors, one of which can be split into multiple spectral detecotrs. The LSM980 can achieve non-diffraction limited optical sectioning with resolutions down to 140nm. The AiryScan2 also allows acquisition in “Multiplexing” mode(s), which accelerates the acquisition speed (up to 8x faster than normal confocal) and a sensitivity mode that enhances the signal-to-noise acquisition. The microscope is also equipped with a dark heating and athmospheric control chamber to allow live imaging of mammalian cells.
The Deltavision OMX is a structured illumination super-resolution microscope that allows the visualization of structures or objects bellow the theoretical limit of resolution. This microscope technique uses the structured illumination approach which is simple to use and gives fast results with minimal to medium processing, allowing users to obtain super resolution images with ease. The resolution limit of this technique is about half the theoretical limit of conventional microscopes at about 100nm.
Light Sheet Microscopy (LSM) is a fluorescence microscopy technique, where the illumination is done perpendicularly to the detection. The technique shapes the illumination laser beam into a rectangle and then focuses it down only in one direction, using a cylindrical lens (SPIM) or galvanometric mirrors (DSLM). This forms a thin "sheet of light" right in the focal plane of the detection objective, illuminating the whole sample plane at the same time. A CMOS camera records the fluorescent signal. This allows obtaining images of a big area in a fast way with a good sectioning of the sample and out-of-focus light suppression. LSM is especially well suited for the investigation of the development of large samples to study features (such as gene expression patterns) that require high resolution while being extended over a large volume and a long period of time. It has been successfully used to track developmental processes on Zebra fish, Drosophila fly, C. elegans nematodes or Arabidopsis plants among others.
The OPenT microscope is a Optical Projection Tomography system which allows to see big samples such as whole organs or embryos without having to open them or taking the organ out. Samples need to be clear and transparent. This system works basically as a CT scan but uses light instead of x-rays for the image acquisition. For more details, advantages and limitations, please speak with the Advanced Imaging personnel.