The Advanced Imaging Lab currently has a few microscopes divided in three categories.
Zeiss' Stereo Lumar is a flurescence capable and fully automated stereoscope with an increased focusing range, letting you take pictures of tissues of whole adult fish, mice and even larger animals. With it's high resolution, high quality ApoLumar 1,2 lens, variable zoom and large working distance users have flexibility to choose between their desired zoom without sacrificing quality.
The Aequoria MDS system is a macroscopic system to aquire luminescence in large samples like living large organisms, bacterial plates, plants or well plates, making it suitable for high-throughput screens. With an EMCCD chip it can detect the smallest signals on your samples with ease.
The DeltaVision is a fully automated research microscope that offers high sensitivity to fluorescence and interference contrast (DIC). With an EM-CCD camera, fully motorized stage (XYZ) and filters, as well as a controlled atmosphere box, coupled with excellent optics it provides excellent conditions for live imaging of cells. The DeltaVision system is also very tightly controlled to provide post-acquisition optical sectioning through deconvolution algorithms; it is also very good for quantitative image-processing.
The Leica DMRA2 is a fully automated research microscope that offers high sensitivity to fluorescence and interference contrast (DIC). The powerful image-processing Leica DMRA2 software operates the microscope with precision.
The Leica DMLB2 is an upright microscope for experiments with Brightfield and DIC.
The Nikon E400 is an upright microscope for experiments with Brightfield and Phase Contrast. Phase contrast is a technique to improve contrast in thin samples. The Nikon E400 has been coupled with a color camera to be able to record images of histology preparations, cells and small organisms.
The Nikon Eclipse TE2000-S is an inverted microscope for experiments with Brightfield and widefield fluorescence. Equipped with a Prior automatic stage, the screening microscope is optimized for cell counting and image stitching, allowing the display of whole samples.
Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample. The Leica SP5 confocal microscope is equipped with a spectral head that employs a prism, movable slits, mirrors, and computer control to permit the operator to choose which bands of light at specific wavelengths will be focused simultaneously onto each of three photomultiplier detectors. This system also allows emission spectra (with 5-nm resolution) to be collected from a diffraction-limited-size spot.
Another SP5, with a few notable differences. Besides having an upright configuration, it is equiped with very sensitive Hybrid detectors and a resonant scanner, making it ideal for confocal live imaging.
The LSM 510 META system is a point scanning laser confocal microscope with spectral analysis suitable for live cell imaging and photobleaching experiments. The system is currently equipped with a large size incubator with high-volume for warm air incubation and CO2 control, ensuring optimal conditions for live imaging. Due to its excellent resolution, high power lasers and extended filter sets it is one of the most used microscope in the institute.
Spinning disk confocal microscopy offers several advantages over conventional optical microscopy, including widefield and laser scanning confocal microscopy. This confocal technique allows acquisition of images at very high frame rates with minimum illumination of samples. These qualities make Spinning Disk confocal microscopy particularly well suited to high speed 3D imaging of living systems.
This confocal microscope allows acquisition of images at very high frame rates with minimum illumination of samples in a broader field of view, when compared with other spinning disks.
Light Sheet Microscopy (LSM) is a fluorescence microscopy technique, where the illumination is done perpendicularly to the detection. The technique shapes the illumination laser beam into a rectangle and then focuses it down only in one direction, using a cylindrical lens (SPIM) or galvanometric mirrors (DSLM). This forms a thin "sheet of light" right in the focal plane of the detection objective, illuminating the whole sample plane at the same time. A CMOS camera records the fluorescent signal. This allows obtaining images of a big area in a fast way with a good sectioning of the sample and out-of-focus light suppression. LSM is especially well suited for the investigation of the development of large samples to study features (such as gene expression patterns) that require high resolution while being extended over a large volume and a long period of time. It has been successfully used to track developmental processes on Zebra fish, Drosophila fly, C. elegans nematodes or Arabidopsis plants among others.
A multi-photon, or two-photon, microscope uses instrumentation similar to that of a laser scanning confocal microscope: A laser source for sample excitation, a scanhead with galvanometer controlled mirrors (or acousto-optic deflectors) to scan the excitation beam, and photomultiplier tubes to detect fluorescent signals. However, the confocal image differs in that optical sectioning is obtained using excitation in multi-photon microscopy whereas in confocal microscopy it is achieved using the emission pinhole...