IGC

Quality control - PSF

Due to the complexity, number and importance of the microscopes available, the Advanced Imaging unit runs regularly scheduled quality control checks on all mission critical microscopes. We will be making these regularly available on this page.

We measure:

  • The resolution of each objective by measuring the apparent size (FWHM - Full Width Half Maximum size) of sub-diffraction single color microspheres of 0.17µm.
  • The Point Spread Function (PSF) of the same microspheres is used to control for aberrations in the image.
  • Colocalization quality of each objective and filter combination using multiply labeled 0.5µm microspheres (still gathering data!).
  • Power output of the lamp/laser at the objective.

Below you can find current results for PSF resolution measurements (in µm).


Leica DMRA2

Note:
Red line - Theoretical XY (0.641 µm)
Orange line - Theoretical Z (4.200 µm)

Note:
Red line - Theoretical XY (0.427 µm)
Orange line - Theoretical Z (1.867 µm)

Note:
Red line - Theoretical XY (0.229 µm)
Orange line - Theoretical Z (0.536 µm)

Note:
Red line - Theoretical XY (0.229 µm)
Orange line - Theoretical Z (0.536 µm)

Deltavision

Note:
Red line - Theoretical XY (0.427 µm)
Orange line - Theoretical Z (1.867 µm)

Note:
Red line - Theoretical XY (0.246 µm)
Orange line - Theoretical Z (0.621 µm)

Note:
Red line - Theoretical XY (0.226 µm)
Orange line - Theoretical Z (0.521 µm)

Note:
Red line - Theoretical XY (0.267 µm)
Orange line - Theoretical Z (0.729 µm)

Note:
Red line - Theoretical XY (0.229 µm)
Orange line - Theoretical Z (0.536 µm)

Leica HCS

Note:
Red line - Theoretical XY (0.458 µm)
Orange line - Theoretical Z (2.143 µm)

Note:
Red line - Theoretical XY (0.246 µm)
Orange line - Theoretical Z (0.621 µm)

Note:
Red line - Theoretical XY (0.229 µm)
Orange line - Theoretical Z (0.536 µm)

Note:
Red line - Theoretical XY (0.215 µm)
Orange line - Theoretical Z (0.473 µm)

Leica SP5 Inverted

Note:
Red line - Theoretical XY (0.300 µm)
Orange line - Theoretical Z (1.500 µm)

Note:
Red line - Theoretical XY (0.300 µm)
Orange line - Theoretical Z (1.500 µm)

Note:
Red line - Theoretical XY (0.247 µm)
Orange line - Theoretical Z (1.017 µm)

Note:
Red line - Theoretical XY (0.168 µm)
Orange line - Theoretical Z (0.247 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Leica SP5 Live

Note:
Red line - Theoretical XY (0.300 µm)
Orange line - Theoretical Z (1.500 µm)

Note:
Red line - Theoretical XY (0.162 µm)
Orange line - Theoretical Z (0.435 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Andor Spinning Disk

Note:
Red line - Theoretical XY (0.280 µm)
Orange line - Theoretical Z (1.307 µm)

Note:
Red line - Theoretical XY (0.162 µm)
Orange line - Theoretical Z (0.435 µm)

Note:
Red line - Theoretical XY (0.175 µm)
Orange line - Theoretical Z (0.510 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Andor Spinning Disk W1 - Ixon Ultra 888

Note:
Red line - Theoretical XY (0.280 µm)
Orange line - Theoretical Z (1.307 µm)

Note:
Red line - Theoretical XY (0.162 µm)
Orange line - Theoretical Z (0.435 µm)

Note:
Red line - Theoretical XY (0.175 µm)
Orange line - Theoretical Z (0.510 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Andor Spinning Disk W1 - Zyla

Note:
Red line - Theoretical XY (0.280 µm)
Orange line - Theoretical Z (1.307 µm)

Note:
Red line - Theoretical XY (0.162 µm)
Orange line - Theoretical Z (0.435 µm)

Note:
Red line - Theoretical XY (0.175 µm)
Orange line - Theoretical Z (0.510 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Note:
Red line - Theoretical XY (0.150 µm)
Orange line - Theoretical Z (0.375 µm)

Roper TIRF

Note:
Red line - Theoretical XY (0.162 µm)
Orange line - Theoretical Z (0.435 µm)

Note:
Red line - Theoretical XY (0.233 µm)
Orange line - Theoretical Z (0.907 µm)

Note:
Red line - Theoretical XY (0.141 µm)
Orange line - Theoretical Z (0.331 µm)