The goal of the Transgenics Unit (TU) is to help research groups at the IGC by generating genetically modified animals required for their research activities. Currently, our services include the introduction of a wide spectrum of genetic modifications into the mouse and Drosophila genomes.
In all cases, the researchers provide the DNA constructs, gRNAs or ES cells. The TU staff then performs all microinjections and surgical procedures required for the production of the mutant mice. We deliver founders or chimeras that are then further crossed and analyzed by the requesting researchers.
The researchers provide the constructs to be injected and we deliver either adult flies resulting from the injections, leaving to the researchers the production of stable transformants, or, with additional costs, we also deliver stable transformants to the user, either as homozygous or balanced.
We work together with the researchers throughout the different stages of the project to make sure that we produce genetically modified animals that best meet the specific requirements of their research activities. We discuss with the researchers the different alternatives and help them to select the best strategy for their specific experimental approach. In addition, we advise on the design of the transgenic and targeting constructs, supply basic reagents that facilitate their production and provide guidance on the production of stable transformants and screening strategies that simplify future work with the mutant strains.
Our main goal is to cover the needs of the research groups at the IGC but under specific circumstances we can also produce genetically modified animals for external users.